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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- sc-5581 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-5581, RRID:AB_2243005
- Product name
- Anti-ARNT2
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Mutant huntingtin fragment selectively suppresses Brn-2 POU domain transcription factor to mediate hypothalamic cell dysfunction.
The Problem of the Two Types of Diamond
Yamanaka T, Tosaki A, Miyazaki H, Kurosawa M, Furukawa Y, Yamada M, Nukina N
Human molecular genetics 2010 Jun 1;19(11):2099-112
Human molecular genetics 2010 Jun 1;19(11):2099-112
The Problem of the Two Types of Diamond
G. B. B. M. SUTHERLAND, D. E. BLACKWELL, W. G. SIMERAL
Nature 1954 Nov;174(4437):901-904
Nature 1954 Nov;174(4437):901-904
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 79
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human hippocampus shows distinct nuclear positivity in neuronal cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
