F50622

antibody from NSJ Bioreagents

Targeting: FGFR4 CD334, JTK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F50622

Provider

NSJ Bioreagents

Product name

Fgfr4 Antibody

Antibody type

Polyclonal

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FGFR4 antibody western blot analysis in 293 lysate. Observed molecular weight: 88~125 kDa depending on phosphorylation and glycosylation level.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent confocal image of HeLa cells stained with FGFR4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with primary Ab (1:200, 2 h at room temperature). For secondary Ab, Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of FGFR4 to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000160867).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human hepatocarcinoma tissue stained with the FGFR4 antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of WiDr cells using FGFR4 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.