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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-29596 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGFR4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Huh-7, HepG2, HepG2 membrane extract. Predicted reactivity: Mouse (86%), Rat (87%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.95 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGFR4/CD334 using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a FGFR4/CD334 polyclonal antibody (Product # PA5-29596) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FGFR4 was performed by separating 30 µg of HepG2 whole cell and membrane extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a FGFR4 Polyclonal Antibody (Product # PA5-29596) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
