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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- 600-401-A33 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-A33, RRID:AB_2107593
- Product name
- Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody - 600-401-A33
- Antibody type
- Polyclonal
- Description
- Anti-GAPDH Antibody is directed against human GAPDH protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Rabbit
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
- Storage
- Store Anti-GAPDH antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Handling
- Dry Ice
Submitted references α-Motor neurons are spared from aging while their synaptic inputs degenerate in monkeys and mice.
Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation.
Co-targeting ALK and EGFR parallel signaling in oral squamous cell carcinoma.
Maxwell N, Castro RW, Sutherland NM, Vaughan KL, Szarowicz MD, de Cabo R, Mattison JA, Valdez G
Aging cell 2018 Apr;17(2)
Aging cell 2018 Apr;17(2)
Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation.
Roy K, Jerman S, Jozsef L, McNamara T, Onyekaba G, Sun Z, Marin EP
The Journal of biological chemistry 2017 Oct 27;292(43):17703-17717
The Journal of biological chemistry 2017 Oct 27;292(43):17703-17717
Co-targeting ALK and EGFR parallel signaling in oral squamous cell carcinoma.
Gonzales CB, De La Chapa JJ, Saikumar P, Singha PK, Dybdal-Hargreaves NF, Chavez J, Horning AM, Parra J, Kirma NB
Oral oncology 2016 Aug;59:12-19
Oral oncology 2016 Aug;59:12-19
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's Affinity Purified anti-GAPDH antibody shows detection of a single band at ~39 kDa corresponding to GAPDH (arrowhead) in HeLa cell lysates. The predicted MW of GAPDH is 36 kDa. GAPDH was detected using 125 ng/ml (lane A), 250 ng/ml (lane B) and 500 ng/ml (lane C) concentrations of primary antibody.
- Validation comment
- Western Blot
