Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-A33S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-A33S, RRID:AB_11182910
- Product name
- Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody - 600-401-A33S
- Antibody type
- Polyclonal
- Description
- GAPDH antibody is directed against human GAPDH protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Rabbit
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 1.0 mg/ml
- Storage
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Handling
- Wet Ice
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's Affinity Purified anti-GAPDH antibody shows detection of a single band at ~39 kDa corresponding to GAPDH (arrowhead) in HeLa cell lysates. The predicted MW of GAPDH is 36 kDa. GAPDH was detected using 125 ng/ml (lane A), 250 ng/ml (lane B) and 500 ng/ml (lane C) concentrations of primary antibody.
- Validation comment
- Western Blot