Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R36416 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GRP78 Antibody / BiP / HSPA5
- Antibody type
- Polyclonal
- Description
- This highly specific GRP78 antibody is suitable for use in Western blot/Immunofluorescence/Immunocytochemistry/Immunohistochemistry/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the GRP78 antibody at -20oC.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of A) HepG2 and B) MCF7 cell lysate with GRP78 antibody at 0.1ug/ml. Predicted molecular weight: ~73kDa, but routinely observed at 70-78kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) mouse liver and 2) mouse testis lysate with GRP78 antibody at 0.3ug/ml and 3) rat brain lysate with GRP78 antibody at 0.5ug/ml. Expected molecular weight: 70-78 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC testing of fixed and permeabilized human HeLa cells with GRP78 antibody (green) at 5ug/ml and DAPI nuclear stain (blue).
