PA1-014A-A488

antibody from Invitrogen Antibodies

Targeting: HSPA5 BiP, GRP78

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-014A-A488

Provider

Invitrogen Antibodies

Product name

GRP78 Polyclonal Antibody, Alexa Fluor™ 488

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1014AA488 detects glucose regulated protein/BiP (GRP78) human, rat and mouse samples.

Conjugate

Green dye

Concentration

1 mg/mL

References

Submitted references

Cytokine-induced translocation of GRP78 to the plasma membrane triggers a pro-apoptotic feedback loop in pancreatic beta cells.

Vig S, Buitinga M, Rondas D, Crèvecoeur I, van Zandvoort M, Waelkens E, Eizirik DL, Gysemans C, Baatsen P, Mathieu C, Overbergh L
Cell death & disease 2019 Apr 5;10(4):309

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Depletion of the membrane-fusion regulator Munc18c attenuates caerulein hyperstimulation-induced pancreatitis.

Dolai S, Liang T, Orabi AI, Xie L, Holmyard D, Javed TA, Fernandez NA, Xie H, Cattral MS, Thurmond DC, Thorn P, Gaisano HY
The Journal of biological chemistry 2018 Feb 16;293(7):2510-2522

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Grp78 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Grp78 Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA1-014A-A488) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Green dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Green dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Surface translocation of glucose-regulated protein 78 (GRP78) in beta cells is an early phenomenon, taking place in the early apoptotic cell population. MIN6 cells exposed to cytokines (human interleukin-1beta (50 U/mL), mouse interferon-gamma (250 U/mL), and mouse tumor necrosis factor-alpha (1000 U/mL)) for the indicated time points (4, 8, 16, and 24 h) were stained with the late-apoptosis/necrosis marker DRAQ7-AF700, early apoptosis marker Annexin V-APC (see Results for the interpretation of these combined dyes to discriminate between living, early, and late apoptotic cells), and anti-GRP78-FITC, followed by flow cytometry. a , b Percentage of living ( a ) and early apoptotic ( b ) cell population in control (C) and cytokine (Cyt) exposed MIN6 cells. c , d Percentage of sGRP78-positive cells in the living ( c ) and early apoptotic ( d ) subpopulation. e , f Geometric mean fluorescence intensity (MFI) for sGRP78 in the living ( e ) and early apoptotic ( f ) subpopulation. Data are presented as mean +- SEM ( n = 4) and statistically analyzed by a two-way analysis of variance followed by Sidak posthoc test for multiple group comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001

Conjugate

Green dye