Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-014A-A647 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRP78 Polyclonal Antibody, Alexa Fluor™ 647
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1014AA647 detects glucose regulated protein/BiP (GRP78) human, rat and mouse samples.
- Conjugate
- Red dye
- Concentration
- 1 mg/mL
Submitted references FOXO1 promotes HIV latency by suppressing ER stress in T cells.
Depletion of the membrane-fusion regulator Munc18c attenuates caerulein hyperstimulation-induced pancreatitis.
Vallejo-Gracia A, Chen IP, Perrone R, Besnard E, Boehm D, Battivelli E, Tezil T, Krey K, Raymond KA, Hull PA, Walter M, Habrylo I, Cruz A, Deeks S, Pillai S, Verdin E, Ott M
Nature microbiology 2020 Sep;5(9):1144-1157
Nature microbiology 2020 Sep;5(9):1144-1157
Depletion of the membrane-fusion regulator Munc18c attenuates caerulein hyperstimulation-induced pancreatitis.
Dolai S, Liang T, Orabi AI, Xie L, Holmyard D, Javed TA, Fernandez NA, Xie H, Cattral MS, Thurmond DC, Thorn P, Gaisano HY
The Journal of biological chemistry 2018 Feb 16;293(7):2510-2522
The Journal of biological chemistry 2018 Feb 16;293(7):2510-2522
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Grp78 (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Grp78 Polyclonal Antibody, AlexaFluor 647 conjugate (Product # PA1-014A-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Red dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Red dye
