MAB4846

antibody from R&D Systems

Targeting: HSPA5 BiP, GRP78

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4846

Provider

R&D Systems

Product name

Human GRP78/HSPA5 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects endogenous human GRP78 at 78 kDa in Western blots.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P11021


Isotype

IgG

Antibody clone number

474421

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells.

Chu H, Chan CM, Zhang X, Wang Y, Yuan S, Zhou J, Au-Yeung RK, Sze KH, Yang D, Shuai H, Hou Y, Li C, Zhao X, Poon VK, Leung SP, Yeung ML, Yan J, Lu G, Jin DY, Gao GF, Chan JF, Yuen KY
The Journal of biological chemistry 2018 Jul 27;293(30):11709-11726

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WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response.

Janczar S, Nautiyal J, Xiao Y, Curry E, Sun M, Zanini E, Paige AJ, Gabra H
Cell death & disease 2017 Jul 27;8(7):e2955

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The endoplasmic reticulum chaperone BiP/GRP78 is important in the structure and function of the human cytomegalovirus assembly compartment.

Buchkovich NJ, Maguire TG, Paton AW, Paton JC, Alwine JC
Journal of virology 2009 Nov;83(22):11421-8

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human GRP78/HSPA5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human GRP78/HSPA5 Monoclonal Antibody (Catalog # MAB4846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRP78/HSPA5 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human GRP78/HSPA5 by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GRP78/HSPA5 at approximately 72 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GRP78/HSPA5 Monoclonal Antibody (Catalog # MAB4846). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.