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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-301-F66 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-F66, RRID:AB_2611175
- Product name
- Anti-HSP90 beta (MOUSE) Monoclonal Antibody - 200-301-F66
- Antibody type
- Monoclonal
- Vial size
- 200 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of mouse anti-Hsp90 antibody. Lane 1: A431. Lane 2: A549. Lane 3: HCT116. Lane 4: HeLa. Lane 5: HepG2. Lane 6: HEK293. Lane 7: HL-60. Lane 8: HUVEC. Lane 9: Jurkat. Lane 10: MCF7. Lane 11: PC3. Lane 12: T98G. Lane 13: Rat Brain. Load: 10 µg per lane. Primary antibody: Hsp90 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 84.1 kDa for Hsp90. Other band(s): none.
- Validation comment
- Western Blot
