MA5-17104

antibody from Invitrogen Antibodies

Targeting: ITGB4 CD104

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17104

Provider

Invitrogen Antibodies

Product name

CD104 Monoclonal Antibody (10B10D5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17104 targets ITGB4 in FACS, IHC and WB applications and shows reactivity with Human samples. The MA5-17104 immunogen is purified recombinant fragment of human ITGB4 (amino acids: 1619-1822) expressed in E. Coli. MA5-17104 detects ITGB4 which has a predicted molecular weight of approximately 202kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

10B10D5

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Integrin α6β4E variant is associated with actin and CD9 structures and modifies the biophysical properties of cell-cell and cell-extracellular matrix interactions.

Wang M, Hinton JP, Gard JMC, Garcia JGN, Knudsen BS, Nagle RB, Cress AE
Molecular biology of the cell 2019 Mar 21;30(7):838-850

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD104/Integrin beta 4 using a CD104/Integrin beta 4 monoclonal antibody (Product # MA5-17104) against a human ITGB4 recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD104/Integrin beta 4 monoclonal antibody (Product # MA5-17104) against human CD104/Integrin beta 4 recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD104/Integrin beta 4 using a CD104/Integrin beta 4 monoclonal antibody (Product # MA5-17104) against a human ITGB4 recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD104/Integrin beta 4 using CD104/Integrin beta 4 monoclonal antibody (Product # MA5-17104) in A549 (1), A431 (2), MCF-7 (3) and SW620 (4) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CD104 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD104 Monoclonal Antibody (10B10D5) (Product # MA5-17104) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD104/Integrin beta 4 monoclonal antibody (Product # MA5-17104) with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD104/Integrin beta 4 monoclonal antibody (Product # MA5-17104) with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD104/Integrin beta 4 monoclonal antibody (Product # MA5-17104) followed with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD104/Integrin beta 4 monoclonal antibody (Product # MA5-17104) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of A549 cells using CD104/Integrin beta 4 monoclonal antibody (Product # MA5-17104) (green) and negative control (purple).