Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [3]
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Validation data
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- Product number
- 13-1049-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD104 (Integrin beta 4) Monoclonal Antibody (439-9B), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 439-9B recognizes human CD104, also known as integrin beta 4. CD104 is a 202 kDa subunit that associates with integrin alpha 6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. Expression is found on epithelial cells and tumor cells and has been shown to be involved in cell-matrix and cell-cell interactions. The role of CD104 in tumorigenicity is well established. The monoclonal antibody 439-9B has been reported to have no effect on adhesion of human colon adenocarcinoma cells. Moreover, this antibody crossreacts to bovine. Applications Reported: This 439-9B antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. Applications Tested: This 439-9B antibody has been tested by flow cytometric analysis of EDTA treated A549 cell line. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 439-9B
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Identification of cell context-dependent YAP-associated proteins reveals β(1) and β(4) integrin mediate YAP translocation independently of cell spreading.
Integrin-β4 identifies cancer stem cell-enriched populations of partially mesenchymal carcinoma cells.
Endothelial protein C receptor function in murine and human breast cancer development.
Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin.
Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue.
Lee JY, Dominguez AA, Nam S, Stowers RS, Qi LS, Chaudhuri O
Scientific reports 2019 Nov 20;9(1):17188
Scientific reports 2019 Nov 20;9(1):17188
Integrin-β4 identifies cancer stem cell-enriched populations of partially mesenchymal carcinoma cells.
Bierie B, Pierce SE, Kroeger C, Stover DG, Pattabiraman DR, Thiru P, Liu Donaher J, Reinhardt F, Chaffer CL, Keckesova Z, Weinberg RA
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 21;114(12):E2337-E2346
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 21;114(12):E2337-E2346
Endothelial protein C receptor function in murine and human breast cancer development.
Schaffner F, Yokota N, Carneiro-Lobo T, Kitano M, Schaffer M, Anderson GM, Mueller BM, Esmon CT, Ruf W
PloS one 2013;8(4):e61071
PloS one 2013;8(4):e61071
Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin.
Simon-Assmann P, Leberquier C, Molto N, Uezato T, Bouziges F, Kedinger M
Journal of cell science 1994 Mar;107 ( Pt 3):577-87
Journal of cell science 1994 Mar;107 ( Pt 3):577-87
Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue.
Falcioni R, Sacchi A, Resau J, Kennel SJ
Cancer research 1988 Feb 15;48(4):816-21
Cancer research 1988 Feb 15;48(4):816-21
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the A549 cell line with 0.125 µg of Rat IgG2b K Isotype Control Biotin (Product # 13-4031-82) (open histogram) or 0.125 µg of Anti-Human CD104 (Integrin beta 4) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 beta 1 and beta 4 integrin KO decrease YAP nuclear localization. ( a) Western blot analysis of MCF10A DeltaITGB1 and DeltaITGB4 cells compared to control DeltaGAL4 cells. GAPDH was used as a loading control. Quantification of bands (beta 1 or beta 4 integrin/GAPDH) below each lane. Images cropped from the same blot using different fluorescent intensities. Full-length blot included in Supplementary Fig. 2 . (b) DeltaITGB1 cells stained for beta 1 integrin (green) and YAP (red), DNA stained with DAPI (blue). (c) DeltaITGB4 cells stained for beta 4 integrin (green) and YAP (red), DNA stained with DAPI (blue). (d) YAP nuclear localization in KO cells, **p < 0.01. (e) Nuclear and cytoplasmic areas of KO cells, **p < 0.002. Bars represent mean +- SEM, symbols represent each cell, p-values from one-way ANOVA followed by Tukey post-hoc comparison tests. (f) DeltaGAL4 YAP nuclear localization with area. (g) DeltaITGB1 YAP nuclear localization with area. (h) DeltaITGB4 YAP nuclear localization with area. N = 24-32 cells from 3 independent experiments. (i) Comparison of fits for YAP nuclear localization with area in KO cells. Comparison of (j) Slopes and (k) y-intercepts from best fit lines of YAP nuclear localization with area in KO cells from ( f - h ). **p < 0.01. p-values from linear regression comparing 3 KO best fit lines.
- Conjugate
- Biotin