Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-1049-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD104 (Integrin beta 4) Monoclonal Antibody (439-9B), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 439-9B recognizes human CD104, also known as integrin beta 4. CD104 is a 202 kDa subunit that associates with integrin alpha 6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. Expression is found on epithelial cells and tumor cells and has been shown to be involved in cell-matrix and cell-cell interactions. The role of CD104 in tumorigenicity is well established. The monoclonal antibody 439-9B has been reported to have no effect on adhesion of human colon adenocarcinoma cells. Moreover, this antibody crossreacts to bovine. Applications Reported: This 439-9B antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting under nonreducing conditions only, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. Applications Tested: This 439-9B antibody has been tested by flow cytometric analysis of A549 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 439-9B
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Integrin-β4 identifies cancer stem cell-enriched populations of partially mesenchymal carcinoma cells.
Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma.
Upregulation of integrin β4 promotes epithelial-mesenchymal transition and is a novel prognostic marker in pancreatic ductal adenocarcinoma.
Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin.
Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue.
Bierie B, Pierce SE, Kroeger C, Stover DG, Pattabiraman DR, Thiru P, Liu Donaher J, Reinhardt F, Chaffer CL, Keckesova Z, Weinberg RA
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 21;114(12):E2337-E2346
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 21;114(12):E2337-E2346
Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma.
Nguyen N, Bellile E, Thomas D, McHugh J, Rozek L, Virani S, Peterson L, Carey TE, Walline H, Moyer J, Spector M, Perim D, Prince M, McLean S, Bradford CR, Taylor JM, Wolf GT, Head and Neck SPORE Program Investigators.
Head & neck 2016 Jul;38(7):1074-84
Head & neck 2016 Jul;38(7):1074-84
Upregulation of integrin β4 promotes epithelial-mesenchymal transition and is a novel prognostic marker in pancreatic ductal adenocarcinoma.
Masugi Y, Yamazaki K, Emoto K, Effendi K, Tsujikawa H, Kitago M, Itano O, Kitagawa Y, Sakamoto M
Laboratory investigation; a journal of technical methods and pathology 2015 Mar;95(3):308-19
Laboratory investigation; a journal of technical methods and pathology 2015 Mar;95(3):308-19
Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin.
Simon-Assmann P, Leberquier C, Molto N, Uezato T, Bouziges F, Kedinger M
Journal of cell science 1994 Mar;107 ( Pt 3):577-87
Journal of cell science 1994 Mar;107 ( Pt 3):577-87
Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue.
Falcioni R, Sacchi A, Resau J, Kennel SJ
Cancer research 1988 Feb 15;48(4):816-21
Cancer research 1988 Feb 15;48(4):816-21
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the A549 cell line with 0.5 µg of Rat IgG2b K Isotype Control Purified (Product # 14-4031-82) (open histogram) or 0.5 µg of Anti-Human CD104 (Integrin beta 4) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85)and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.