Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 50-1049-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD104 (Integrin beta 4) Monoclonal Antibody (439-9B), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 439-9B recognizes human CD104, also known as integrin beta 4. CD104 is a 202 kDa subunit that associates with integrin alpha 6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. Expression is found on epithelial cells and tumor cells and has been shown to be involved in cell-matrix and cell-cell interactions. The role of CD104 in tumorigenicity is well established. The monoclonal antibody 439-9B has been reported to have no effect on adhesion of human colon adenocarcinoma cells. Moreover, this antibody crossreacts to bovine. Applications Reported: This 439-9B antibody has been reported for use in flow cytometric analysis. Applications Tested: This 439-9B antibody has been tested by flow cytometric analysis of A549 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 439-9B
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Integrin-β4 identifies cancer stem cell-enriched populations of partially mesenchymal carcinoma cells.
Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin.
Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Integrin-β4 identifies cancer stem cell-enriched populations of partially mesenchymal carcinoma cells.
Bierie B, Pierce SE, Kroeger C, Stover DG, Pattabiraman DR, Thiru P, Liu Donaher J, Reinhardt F, Chaffer CL, Keckesova Z, Weinberg RA
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 21;114(12):E2337-E2346
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 21;114(12):E2337-E2346
Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin.
Simon-Assmann P, Leberquier C, Molto N, Uezato T, Bouziges F, Kedinger M
Journal of cell science 1994 Mar;107 ( Pt 3):577-87
Journal of cell science 1994 Mar;107 ( Pt 3):577-87
Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue.
Falcioni R, Sacchi A, Resau J, Kennel SJ
Cancer research 1988 Feb 15;48(4):816-21
Cancer research 1988 Feb 15;48(4):816-21
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of A549 cells with 0.5 µg of Rat IgG2b Isotype Control eFluor® 660 (Product # 50-4031-82) (blue histogram) or 0.5 µg of Anti-Human CD104 (Integrin beta 4) eFluor® 660 (purple histogram). Total viable cells were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL