Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB28416 - Provider product page
- Provider
- Abnova Corporation
- Product name
- MECP2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant MECP2.
- Antigen sequence
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDK
KEEKEGKHEPVQPSAHHSAEPAGAGKAETSEGSGS
APAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWT
RKLKQRKSGRSAGKY- Isotype
- IgG
- Storage
- Store at 4 °C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of HEK293T cell lysate using MECP2 polyclonal antibody (Cat # PAB28416).Lane 1: Negative control (vector only)Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human cerebral cortex with MECP2 polyclonal antibody (Cat # PAB28416) shows strong nuclear positivity in neuronal and glial cells.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)