Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX60358 - Provider product page
- Provider
- GeneTex
- Product name
- MECP2 antibody - ChIP grade
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of nuclear extracts (40 £gg) from HeLa cells using MeCP2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of MeCP2 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with U2OS nuclear extract and either 5 £gg of H3K79me2 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to £]-Actin or Sat2.
