H00008738-M01

antibody from Abnova Corporation

Targeting: CRADD RAIDD

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008738-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008738-M01, RRID:AB_437082

Product name

CRADD monoclonal antibody (M01), clone 1F8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CRADD.

Antigen sequence

MEARDKQVLRSLRLELGAEVLVEGLVLQYVYEEGI
LTENHIQEINAQTTGLRKTMLLLDILPSRGPKAFD
TFLDSLQEFPWVREKLKKAREEAMTDLPAGDRLTG
IPSHILNSSPSDRQINQLAQRLGPEWEPMVLSLGL
SQTDIYRCKANHPHNVQSQVVEAFIRWRQRFGKQA
TFQSLHNGLRAVEVDPSLLLHMLE


Isotype

IgG

Antibody clone number

1F8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CRADD monoclonal antibody (M01), clone 1F8 Western Blot analysis of CRADD expression in K-562 ( Cat # L009V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CRADD expression in transfected 293T cell line by CRADD monoclonal antibody (M01), clone 1F8.Lane 1: CRADD transfected lysate(23 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CRADD is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to CRADD on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol