PA1-32166
antibody from Invitrogen Antibodies
Targeting: PTPN11
BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- PA1-32166 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SHP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX29214-PEP).. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SHP2 in human muscle lysate (RIPA buffer, 35 µg total protein per lane) using a SHP2 polyclonal antibody (Product # PA1-32166) at a dilution of 2 µg/mL following incubation for 1 hour and detected using chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SHP2 in HEK293 overexpressing PTPN11 (mock transfection in first lane) using a SHP2 polyclonal antibody (Product # PA1-32166).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SHP2 was performed by loading 35 µg (in RIPA buffer) of human muscle lysates. Proteins were transferred to a membrane and probed with a SHP2 Polyclonal Antibody (Product # PA1-32166) at a dilution of 2 µg/mL.