PA5-17722

antibody from Invitrogen Antibodies

Targeting: PTPN11 BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2

Antibody data

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Validation data

PTPN11 protein structure - PA5-17722 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SHP2 was achieved by transfecting A-431 with SHP2 specific siRNAs (Silencer® select Product # s11525, s11526). Western blot analysis (Fig. a) was performed using Whole cell extracts from the SHP2 knockdown cells (lane 2) and non-targeting scrambled siRNA transfected cells (lane 1). The blot was probed with SHP2 Polyclonal Antibody (Product # PA5-17722, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SHP2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-SHP2 Polyclonal Antibody (Product # PA5-17722) and a 68kDa band corresponding to SHP2 was observed across cell lines and tissue tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), HT-29 (Lane 2), HeLa (Lane 3), HEK-293 (Lane 4), C2C12 (Lane 5), Mouse Brain (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).