Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AP3716a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP3716a, RRID:AB_10664552
- Product name
- Phospho-SHP2(Y584) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Dot blot analysis of anti-Phospho-SHP2-pY584 Phospho-specific Pab (Cat. #AP3716a ) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml."
- Primary Ab dilution
- 1:500
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of lysate from NIH-3T3 cells(from left to right),untreated or treated with PDGF at 50ng/ml,using Phospho-SHP2(Y584) Antibody (Cat. #AP3716a) or SHP2 Antibody.Lysate at 15ug per lane.AP3716a was diluted at 1:2000 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody."
- Primary Ab dilution
- 1:2000