AP3716a

antibody from Abgent

Targeting: PTPN11 BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP3716a

Provider

Abgent

Proper citation

Abgent Cat#AP3716a, RRID:AB_10664552

Product name

Phospho-SHP2(Y584) Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Dot blot analysis of anti-Phospho-SHP2-pY584 Phospho-specific Pab (Cat. #AP3716a ) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml."

Primary Ab dilution

1:500

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of lysate from NIH-3T3 cells(from left to right),untreated or treated with PDGF at 50ng/ml,using Phospho-SHP2(Y584) Antibody (Cat. #AP3716a) or SHP2 Antibody.Lysate at 15ug per lane.AP3716a was diluted at 1:2000 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody."

Primary Ab dilution

1:2000