AP8471a

antibody from Abgent

Targeting: PTPN11 BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP8471a

Provider

Abgent

Proper citation

Abgent Cat#AP8471a, RRID:AB_637452

Product name

SHP2 Antibody

Antibody type

Polyclonal

Antigen

Recombinant Protein

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PTPN11 (arrow) using rabbit polyclonal SHP2 Antibody (RB07283). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN11 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent image of Hela cell stained with SHP2 Antibody(Cat#AP8471a/SH050329B).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SHP2 primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!).SHP2 immunoreactivity is localized to Nucleolus and Cytoplasm significantly and Nucleus weakly."

Primary Ab dilution

1:10~50