20145-1-AP

antibody from Proteintech Group

Targeting: PTPN11 BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2

Provider product page for 20145-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [45]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 20145-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20145-1-AP, RRID:AB_10699877
Product name: SHP2 antibody
Antibody type: Polyclonal
Description: KD/KO validated SHP2 antibody (Cat. #20145-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PTPN11 protein structure - 20145-1-AP shown in red.

Submitted references SHP2 improved Late-onset fetal growth restriction via modulating ROS/BRD4/PI3K/YAP/PIGF signaling induced angiogenesis.

Li F, Li Y, Hao W, Zhang X, Shen X, Yang B, Zhang W, Fu L, Gu S
PloS one 2026;21(2):e0342649

A novel proteolysis-targeting chimera strategy targeting multiple immune checkpoints containing ITIMs enhances antitumor immunity.

Qiu YY, Wang ZW, He L, Shi GG, Li ZZ, Ma SX, Yu D, Du HC, Xie F, Zhang C, Zhang YQ, Li M, Li WN
Journal of pharmaceutical analysis 2026 Mar;16(3):101511

Signal Transducer Nanoparticles Enable Siglec-10/G Blockade Immunotherapy for Breast Cancer Treatment.

Meng Z, Gao X, Tang X, Fan J, Sheu WC, Tu Z, Yu J, Long G, Wang L, Liu R, Zhou J
Advanced materials (Deerfield Beach, Fla.) 2025 Oct;37(39):e2502758

LILRB4 exacerbates myocardial ischemia-reperfusion injury by promoting inflammation and pyroptosis.

Yang J, Zhang M, Liu L, Zhang P, Liu H, Yang Z, Chen C, Li R, Zhai Y, Li W, Wang W, Yang J, Huang C, Zhang J
Biochimica et biophysica acta. Molecular basis of disease 2025 Oct;1871(7):167959

SHP2-mediated ROS activation induces chondrocyte paraptosis in osteoarthritis and is attenuated by low-intensity pulsed ultrasound.

Hou W, Shang X, Hao X, Pan C, Zheng Z, Zhang Y, Deng X, Chi R, Liu J, Guo F, Sun K, Xu T
Journal of orthopaedic translation 2025 May;52:233-248

Inhibiting SHP2 reduces glycolysis, promotes microglial M1 polarization, and alleviates secondary inflammation following spinal cord injury in a mouse model.

Ding X, Chen C, Zhao H, Dai B, Ye L, Song T, Huang S, Wang J, You T
Neural regeneration research 2025 Mar 1;20(3):858-872

Sea Cucumber Fucoidan Inhibits Helicobacter pylori Gastritis via MAPK/NF-κB Signaling and Gut Microbiota Modulation.

Cao J, Yao M, Wang K, Qin L, Zhang Q, Zhang H, Wei J, Qu C, Xue C, Miao J
Journal of agricultural and food chemistry 2025 Jun 11;73(23):14333-14352

Protein kinase a suppresses antiproliferative effect of interferon-α in hepatocellular carcinoma by activation of protein tyrosine phosphatase SHP2.

Sheng Y, Lin Y, Qiang Z, Shen X, He Y, Li L, Li S, Zhang G, Wang F
The Journal of biological chemistry 2025 Feb;301(2):108195

Shp2 regulates the trophoblast cell cycle progression through p53-p21 pathway modulation.

Meng MH, Wu X, Qin T, Mo WY, Long XG, Li Y, Zhang P, Lu JN, Pan D, Zhang XQ
Biochemical and biophysical research communications 2025 Aug 30;776:152209

Discovery of Novel SHP2 ATTEC Degraders against Pancreatic Ductal Adenocarcinoma Harboring KRAS(G12D) Mutations.

Zhong Y, Lu Y, Li J, Ren Q, Fan Y, Meng X, Shao J, Qian H
Journal of medicinal chemistry 2025 Apr 24;68(8):8143-8162

Protective effects and mechanism of Sangyu granule on acetaminophen-induced liver injury in mice.

Xiao K, Li H, Li Y, Zhan B, Fang X, Zhao B, Zhang X, Wu Y, Wang F, Jia Y
Journal of ethnopharmacology 2024 Sep 15;331:118282

Exploring the anti-inflammatory and immune regulatory effects of Taohe Chengqi decoction in sepsis-induced lung injury.

Deng M, Chen S, Wu J, Su L, Xu Z, Jiang C, Sheng L, Yang X, Zeng L, Wang J, Dai W
Journal of ethnopharmacology 2024 Oct 28;333:118404

Baliosperoid A attenuates lipopolysaccharide-induced acute lung injury by targeting SHP2 to inhibit inflammation and oxidative stress.

Li Y, Zhao L, Liu Z, Chen Y, Li X, Zhu D, Liu L
Bioorganic chemistry 2024 Dec;153:107982

Allosteric inhibition of the tyrosine phosphatase SHP2 enhances the anti-tumor immunity of interferon α through induction of caspase-1-mediated pyroptosis in renal cancer.

Xi R, Cao Y, Fu N, Sheng Y, Yu J, Li L, Zhang G, Wang F
International immunopharmacology 2024 Dec 25;143(Pt 2):113498

Rajala A, Rajala R, Bhat MA, Eminhizer M, Hao J, Du J, Rajala RVS
Cell death & disease 2024 Aug 8;15(8):577

Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma.

Pucci P, Lee LC, Han M, Matthews JD, Jahangiri L, Schlederer M, Manners E, Sorby-Adams A, Kaggie J, Trigg RM, Steel C, Hare L, James ER, Prokoph N, Ducray SP, Merkel O, Rifatbegovic F, Luo J, Taschner-Mandl S, Kenner L, Burke GAA, Turner SD
Nature communications 2024 Apr 23;15(1):3422

Resveratrol Enhances Temozolomide Efficacy in Glioblastoma Cells through Downregulated MGMT and Negative Regulators-Related STAT3 Inactivation.

Wu M, Song D, Li H, Ahmad N, Xu H, Yang X, Wang Q, Cheng X, Deng S, Shu X
International journal of molecular sciences 2023 May 29;24(11)

Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance.

Gao X, Wu Y, Chick JM, Abbott A, Jiang B, Wang DJ, Comte-Walters S, Johnson RH, Oberholtzer N, Nishimura MI, Gygi SP, Mehta A, Guttridge DC, Ball L, Mehrotra S, Sicinski P, Yu XZ, Wang H
Cell reports 2023 Apr 25;42(4):112314

miR-6071 inhibits hepatocellular carcinoma progression via targeting PTPN11.

Chen M, Wang H, Shi S, Zhang H, Xu S, Jiang Y
Archives of biochemistry and biophysics 2022 Sep 30;727:109345

A novel 2B4 receptor leads to worse pregnancy outcomes by facilitating TNF-α and IFN-γ production in dNK cells during Toxoplasma gondii infection.

Xu X, Zheng G, Ren Y, He X, Peng B, Hu X, Liu W
Parasites & vectors 2022 Sep 24;15(1):337

Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells.

Zhang R, Chen D, Fan H, Wu R, Tu J, Zhang FQ, Wang M, Zheng H, Qu CK, Elf SE, Faubert B, He YY, Bissonnette MB, Gao X, DeBerardinis RJ, Chen J
Cell chemical biology 2022 Jul 21;29(7):1200-1208.e6

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Chu H, Zhao Q, Liu J, Yang K, Wang Y, Liu J, Zhang K, Zhao B, He H, Zheng Y, Zhong S, Liang Z, Zhang L, Zhang Y
Analytical chemistry 2022 Jan 18;94(2):758-767

Structure-based discovery of a specific SHP2 inhibitor with enhanced blood-brain barrier penetration from PubChem database.

Ma Y, Li WY, Sun T, Zhang L, Lu XH, Yang B, Wang RL
Bioorganic chemistry 2022 Apr;121:105648

Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways.

Fu NJ, Xi RY, Shi XK, Li RZ, Zhang ZH, Li LY, Zhang GL, Wang F
Toxicology and applied pharmacology 2022 Apr 15;441:115988

Inhibition of Phosphodiesterase 5 Promotes the Aromatase-Mediated Estrogen Biosynthesis in Osteoblastic Cells by Activation of cGMP/PKG/SHP2 Pathway.

Wisanwattana W, Wongkrajang K, Cao DY, Shi XK, Zhang ZH, Zhou ZY, Li F, Mei QG, Wang C, Suksamrarn A, Zhang GL, Wang F
Frontiers in endocrinology 2021;12:636784

Brevilin A Inhibits STAT3 Signaling and Induces ROS-Dependent Apoptosis, Mitochondrial Stress and Endoplasmic Reticulum Stress in MCF-7 Breast Cancer Cells.

Saleem MZ, Nisar MA, Alshwmi M, Din SRU, Gamallat Y, Khan M, Ma T
OncoTargets and therapy 2020;13:435-450

Inhibition of JNK-Mediated Autophagy Promotes Proscillaridin A- Induced Apoptosis via ROS Generation, Intracellular Ca(+2) Oscillation and Inhibiting STAT3 Signaling in Breast Cancer Cells.

Saleem MZ, Alshwmi M, Zhang H, Din SRU, Nisar MA, Khan M, Alam S, Alam G, Jin L, Ma T
Frontiers in pharmacology 2020;11:01055

Shp2 activation in bone marrow microenvironment mediates the drug resistance of B-cell acute lymphoblastic leukemia through enhancing the role of VCAM-1/VLA-4.

Yu K, Yin Y, Ma D, Lu T, Wei D, Xiong J, Zhou Z, Zhang T, Zhang S, Fang Q, Wang J
International immunopharmacology 2020 Mar;80:106008

miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance.

Han T, Zhang Y, Yang X, Han L, Li H, Chen T, Zheng Z
Molecular therapy. Nucleic acids 2020 Mar 6;19:1073-1085

The role of tyrosine phosphatase Shp2 in spermatogonial differentiation and spermatocyte meiosis.

Li Y, Liu WS, Yi J, Kong SB, Ding JC, Zhao YN, Tian YP, Feng GS, Li CJ, Liu W, Wang HB, Lu ZX
Asian journal of andrology 2020 Jan-Feb;22(1):79-87

Anticancer activity of dietary xanthone α-mangostin against hepatocellular carcinoma by inhibition of STAT3 signaling via stabilization of SHP1.

Zhang H, Tan YP, Zhao L, Wang L, Fu NJ, Zheng SP, Shen XF
Cell death & disease 2020 Jan 24;11(1):63

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.

Shi C, Yang J, Hu L, Liao B, Qiao L, Shen W, Xie F, Zhu G
Aging 2020 Aug 3;12(15):15546-15555

A thiazole-derived oridonin analogue exhibits antitumor activity by directly and allosterically inhibiting STAT3.

Shen X, Zhao L, Chen P, Gong Y, Liu D, Zhang X, Dai L, Sun Q, Lou J, Jin Z, Zhang B, Niu D, Chen C, Qi X, Jia D
The Journal of biological chemistry 2019 Nov 15;294(46):17471-17486

Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.

Patel Y, Soni M, Awgulewitsch A, Kern MJ, Liu S, Shah N, Singh UP, Chen H
Oncogene 2019 Jan;38(3):445-453

Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.

Maryam A, Mehmood T, Yan Q, Li Y, Khan M, Ma T
Oxidative medicine and cellular longevity 2018;2018:3853409

Proscillaridin A induces apoptosis, inhibits STAT3 activation and augments doxorubicin toxicity in prostate cancer cells.

He Y, Khan M, Yang J, Yao M, Yu S, Gao H
International journal of medical sciences 2018;15(8):832-839

Chemical and genetic inhibition of STAT3 sensitizes hepatocellular carcinoma cells to sorafenib induced cell death.

Xie L, Zeng Y, Dai Z, He W, Ke H, Lin Q, Chen Y, Bu J, Lin D, Zheng M
International journal of biological sciences 2018;14(5):577-585

The abnormal activation of D1R/Shp-2 complex involved in levodopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson's rats.

Wu N, Wan Y, Song L, Qi C, Liu Z, Gan J
Neuropsychiatric disease and treatment 2018;14:1779-1786

Levodopa/Benserazide Loaded Microspheres Alleviate L-dopa Induced Dyskinesia through Preventing the Over-Expression of D1R/Shp-2/ERK1/2 Signaling Pathway in a Rat Model of Parkinson's Disease.

Wan Y, Wu N, Song L, Wang X, Liu Z, Yuan W, Gan J
Frontiers in aging neuroscience 2017;9:331

Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.

Xiang D, Cheng Z, Liu H, Wang X, Han T, Sun W, Li X, Yang W, Chen C, Xia M, Liu N, Yin S, Jin G, Lee T, Dong L, Hu H, Wang H, Ding J
Hepatology (Baltimore, Md.) 2017 May;65(5):1566-1580

Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation.

Lu L, Zhang S, Li C, Zhou C, Li D, Liu P, Huang M, Shen X
Cell death & disease 2017 May 11;8(5):e2767

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Igbe I, Shen XF, Jiao W, Qiang Z, Deng T, Li S, Liu WL, Liu HW, Zhang GL, Wang F
Oncotarget 2017 Dec 26;8(69):113734-113748

Expression and clinical significance of tyrosine phosphatase SHP2 in thyroid carcinoma.

Hu ZQ, Ma R, Zhang CM, Li J, Li L, Hu ZT, Gao QI, Li WM
Oncology letters 2015 Sep;10(3):1507-1512

Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling.

Xiang M, Su H, Hong Z, Yang T, Shu G
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2015 Jun;80:62-71

Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells.

Hagberg N, Theorell J, Schlums H, Eloranta ML, Bryceson YT, Rönnblom L
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 15;191(6):2989-98

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 20145-1-AP(PTPN11 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using PTPN11 antibody 20145-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 20145-1-AP(PTPN11 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 20145-1-AP(PTPN11 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue