LS-C313006

antibody from LSBio

Targeting: PTPN11 BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2

Western blot (Enhanced validation)

Antibody Data

Product number

LS-C313006

Provider

LSBio

Product name

PTPN11 / SHP-2 / NS1 Antibody (aa582-597) LS-C313006

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Bovine, Chicken/Avian, Guinea Pig, Horse, Porcine, Rabbit, Sheep, Simian, Xenopus

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB of PTPN11 / SHP-2 / NS1 antibody. Lane 1: Rat Heart Tissue Lysate. Lane 2: Rat Skeletal Muscle Tissue Lysate. Lane 3: Rat Brain Tissue Lysate. Lane 4: HELA Cell Lysate. Lane 5: A549 Cell Lysate.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of SHP2 using anti-SHP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Heart Tissue Lysate, Lane 2: Rat Skeletal Muscle Tissue Lysate, Lane 3: Rat Brain Tissue Lysate, Lane 4: HELA Whole Cell Lysate, Lane 5: A549 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68KD. The expected band size for SHP2 is at 68KD.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of SHP2 using anti-SHP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: Rat Heart Tissue Lysate, Lane 4: Rat Skeletal Muscle Tissue Lysate, Lane 5: A431 Whole Cell Lysate, Lane 6: JURKAT Whole Cell Lysate, Lane 7: HELA Whole Cell Lysate, Lane 8: U87 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68KD. The expected band size for SHP2 is at 68KD.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of SHP2 using anti-SHP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Heart Tissue Lysate, Lane 2: Rat Skeletal Muscle Tissue Lysate, Lane 3: Rat Brain Tissue Lysate, Lane 4: HELA Whole Cell Lysate, Lane 5: A549 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68KD. The expected band size for SHP2 is at 68KD.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of SHP2 using anti-SHP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: Rat Heart Tissue Lysate, Lane 4: Rat Skeletal Muscle Tissue Lysate, Lane 5: A431 Whole Cell Lysate, Lane 6: JURKAT Whole Cell Lysate, Lane 7: HELA Whole Cell Lysate, Lane 8: U87 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68KD. The expected band size for SHP2 is at 68KD.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB of PTPN11 / SHP-2 / NS1 antibody. Lane 1: Rat Heart Tissue Lysate. Lane 2: Rat Skeletal Muscle Tissue Lysate. Lane 3: Rat Brain Tissue Lysate. Lane 4: HELA Cell Lysate. Lane 5: A549 Cell Lysate.