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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- GTX101062 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101062, RRID:AB_1951537
- Product name
- SHP2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Gene expression analysis of hypersensitivity to mosquito bite, chronic active EBV infection and NK/T-lymphoma/leukemia.
Washio K, Oka T, Abdalkader L, Muraoka M, Shimada A, Oda M, Sato H, Takata K, Kagami Y, Shimizu N, Kato S, Kimura H, Nishizaki K, Yoshino T, Tsukahara H
Leukemia & lymphoma 2017 Nov;58(11):2683-2694
Leukemia & lymphoma 2017 Nov;58(11):2683-2694
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SHP2 antibody detects PTPN11 protein by Western blot analysis.A. 50 ?g rat brain lysate/extract7.5 % SDS-PAGESHP2 antibody (GTX101062) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE GTX101062 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX101062 diluted at 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and SHP2 knockout (KO) 293T cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP2 antibody (GTX101062) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft , using SHP2(GTX101062) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SHP2 antibody detects PTPN11 protein at cytosol on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. SHP2 antibody (GTX101062) dilution: 1:500.
