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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA302214 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Antibody against PTPN11
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Antibody against PTPN11
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PTPN11
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of PTPN11 (arrow) using rabbit polyclonal SHP2 Antibody (RB07283). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN11 gene (Lane 2) (Origene Technologies).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with SHP2 Antibody(Cat#TA302214/SH050329B).Hela cells were incubated with SHP2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).SHP2 immunoreactivity is localized to Nucleolus and Cytoplasm significantly and Nucleus weakly.
- Validation comment
- IF
