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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- ARG63447 - Provider product page
- Provider
- Arigo
- Product name
- anti-SHP2 / PTPN11 antibody
- Antibody type
- Polyclonal
- Antigen
- C-YENVGLMQQQKSFR
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: human muscle lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63447 anti-SHP2 / PTPN11 antibody at 2 µg/ml dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: 1). Mock transfection; 2) PTPN11 (RC220029) expressing plasmid transfected HEK293 cell lysate standed with ARG63447 anti-SHP2 / PTPN11 antibody
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin embedded Human Cerebellum (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63447 anti-SHP2 / PTPN11 antibody at 3.8 µg/ml dilution followed by AP-staining.
