PA5-48111

antibody from Invitrogen Antibodies

Targeting: PTPN11 BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2

Antibody data

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Validation data

PTPN11 protein structure - PA5-48111 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis from lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 ng/mL human PDGF-BB for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-human/mouse Phospho-SHP-2 (Y542) Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48111), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for Phospho-SHP-2 (Y542) at approximately 72 kDa (as indicated). The lysates were also probed for total SHP-2 with human/mouse/Rat SHP-2 Antigen Affinity-purified Polyclonal Antibody. This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis from lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 ng/mL human PDGF-BB for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-human/mouse Phospho-SHP-2 (Y542) Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48111), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for Phospho-SHP-2 (Y542) at approximately 72 kDa (as indicated). The lysates were also probed for total SHP-2 with human/mouse/Rat SHP-2 Antigen Affinity-purified Polyclonal Antibody. This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-SHP2 (Tyr542) in NIH‚3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 ng/mL Human PDGF-BB for 20 minutes. Samples were incubated in Phospho-SHP2 (Tyr542) polyclonal antibody (Product # PA5-48111) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Rabbit IgG secondary antibody. A specific band was detected for Phospho-SHP‚2 (Y542) at approximately 72 kDa (as indicated). The lysates were also probed for total SHP-2 with Human/Mouse/Rat SHP-2 Antigen Affinity-purified Polyclonal Antibody . This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SHP2 in 0.2 mg/mL lysates of NIH‚3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 ng/mL Recombinant Human PDGF‚BB for 20 minutes. Samples were incubated in SHP2 polyclonal antibody (Product # PA5-48111) using a dilution of 10 µg/mL. A specific band was detected for Phospho-SHP‚2 (Y542) at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.