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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB6037 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6037, RRID:AB_1579362
- Product name
- PTPN11 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of PTPN11.
- Antigen sequence
C-YENVGLMQQQKSFR- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The SH2 tyrosine phosphatase shp2 is required for mammalian limb development.
Saxton TM, Ciruna BG, Holmyard D, Kulkarni S, Harpal K, Rossant J, Pawson T
Nature genetics 2000 Apr;24(4):420-3
Nature genetics 2000 Apr;24(4):420-3
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PTPN11 polyclonal antibody (Cat # PAB6037) staining (2 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing PTPN11 and probed with PTPN11 polyclonal antibody (Cat # PAB6037) (mock transfection in first lane), tested by Origene.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PTPN11 polyclonal antibody (Cat # PAB6037)(3.8 ug/mL) staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
