TA501758

antibody from OriGene

Targeting: PTPN11 BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501758

Provider

OriGene

Proper citation

OriGene Cat#TA501758, RRID:AB_11126425

Product name

PTPN11 (SHP2) mouse monoclonal antibody, clone OTI3F2 (formerly 3F2)

Antibody type

Monoclonal

Description

PTPN11 (SHP2) mouse monoclonal antibody, clone OTI3F2 (formerly 3F2)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

PTPN11

Isotype

IgG

Antibody clone number

OTI3F2

Vial size

100 µl

Concentration

0.99 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN11 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN11 (RC220029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN11.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-PTPN11 antibody(TA501758),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC