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LearnPA5-15594
antibody from Invitrogen Antibodies
Targeting: PTPN11
BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-15594 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SHP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTPN11 (arrow) using a SHP2 polyclonal antibody (Product # PA5-15594) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN11 gene (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTPN11 (arrow) using a SHP2 polyclonal antibody (Product # PA5-15594) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN11 gene (Lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a SHP2 polyclonal antibody (Product # PA5-15594). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a SHP2 polyclonal antibody (Product # PA5-15594) (1:25, 1 hr at 37ºC). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37ºC). Actin filaments have been labeled with dye-conjugated phalloidin (red).
