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LearnPA5-27312
antibody from Invitrogen Antibodies
Targeting: PTPN11
BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-27312 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SHP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, mouse brain, rat brain.
- Concentration
- 0.96 mg/mL
Submitted references Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitment.
Ruiz-Heiland G, Yong JW, von Bremen J, Ruf S
Clinical oral investigations 2021 Apr;25(4):1933-1944
Clinical oral investigations 2021 Apr;25(4):1933-1944
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SHP2 using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SHP2 polyclonal antibody (Product # PA5-27312) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SHP2 Polyclonal Antibody (Product # PA5-27312). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. SHP2 Polyclonal Antibody (Product # PA5-27312) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SHP2 Polyclonal Antibody detects PTPN11 protein by Western blot analysis. A. 50 µg rat brain lysate/extract.7.5 % SDS-PAGE. SHP2 Polyclonal Antibody (Product # PA5-27312) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft , using SHP2 (Product # PA5-27312) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SHP2 Polyclonal Antibody detects PTPN11 protein at cytosol on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. SHP2 Polyclonal Antibody (Product # PA5-27312) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Kinetic analysis performed on OCCM-30 cells after stimulation with leptin. The expression of SHP2; COX2; p-STAT1; STAT1; p-STAT3; STAT3; p-ERK1/2; ERK1/2; p-P38; P38; p-JNK; and JNK were visualized by western blot: Leptin upregulates p-STAT1; p-STAT3; and p-ERK1/2 as well as COX2 on OCCM cells whereas no activation of P38 or JNK was observed. Each experiment was repeated three times
