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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA319240 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal SMAD1 phospho S206 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal SMAD1 phospho S206 antibody
- Reactivity
- Canine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SMAD1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.82 mg/ml
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from HEPG2 (lanes 1-4) derived cell lines treated with PBS, BMP2 (5ng/mL), EGF (1ng/mL), or NaCl for 1h at 37? before harvest. Primary antibody was used at 1:500 and pre-incubated before reacting with blot as follows: top row - with PBS,middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. HRP-conjugated-Rabbit IgG was 1:3,000.
- Validation comment
- WB
