F52570

antibody from NSJ Bioreagents

Targeting: SMAD1 JV4-1, MADH1, MADR1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F52570

Provider

NSJ Bioreagents

Product name

SMAD1 Antibody

Antibody type

Polyclonal

Description

This highly specific SMAD1 antibody is suitable for use in Immunohistochemistry/Immunofluorescence/Western blot/ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the SMAD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from (1) HT-1080, (2) HUVEC, (3) mouse C2C12 cell line and (4) rat liver tissue using SMAD1 antibody at 1:1000. Predicted molecular weight: 52~60 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human heart section using SMAD1 antibody; Ab was diluted at 1:25.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human skeletal muscle section using SMAD1 antibody; Ab was diluted at 1:25 dilution.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human skeletal muscle section using SMAD1 antibody; Ab was diluted at 1:25 dilution.