LS-C352485

antibody from LSBio

Targeting: SMAD1 JV4-1, MADH1, MADR1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C352485

Provider

LSBio

Product name

SMAD1 Antibody (phospho-Ser187) LS-C352485

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Chicken/Avian, Sheep

Host

Rabbit

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of SMAD1 (pS187) expression in HEK293T BMP4-treated (A); HeLa PMA-treated (B); A673 (C); PC12 (D) whole cell lysates.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of SMAD1 (pS187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.