LS-C295416

antibody from LSBio

Targeting: SMAD1 JV4-1, MADH1, MADR1

Immunocytochemistry

Immunoprecipitation

Antibody data

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Validation data

Product number: LS-C295416 - Provider product page
Provider: LSBio
Product name: SMAD1 Antibody (aa270-465) LS-C295416
Antibody type: Polyclonal
Description: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Reactivity: Human, Bovine
Host: Rabbit
Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.

SMAD1 protein structure - LS-C295416 shown in red.

Supportive validation

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western Blot; Sample: Human 293T cell lysate; Primary Ab: 1µg/ml Rabbit Anti-Bovine Smad1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 1µg/ml Rabbit Anti-Bovine Smad1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western Blot; Sample: Human Hela cell lysate; Primary Ab: 1µg/ml Rabbit Anti-Bovine Smad1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Knockout Varification: Lane 1: Wild-type 293T cell lysate; Lane 2: Smad1 knockout 293T cell lysate; Predicted MW: 52kd Observed MW: 60kd Primary Ab: 1µg/ml Rabbit Anti-Bovine Smad1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody