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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09415PU-S - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-SMAD1 Antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-SMAD1 Antibody, Aff - Purified
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Drosophila, Porcine, Sheep
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25ul
- Concentration
- 0.51 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot using Affinity Purified Anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reacting with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRPconjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL.
- Validation comment
- WB
