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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00004086-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004086-M03, RRID:AB_607052
- Product name
- SMAD1 monoclonal antibody (M03), clone 2E9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SMAD1.
- Antigen sequence
MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVD
ALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRS
LDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKP
LECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLV
PRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDS
FQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSS
DPGSPFQMPADTPPPAYLPPEDPMTQDGSQPMDTN
MMAPPLPSEINRGDVQAVAYEEPKHWCSIVYYELN
NRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLS
NVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSD
SSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNN
QEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGW
GAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMG
SPHNPISSVS- Isotype
- IgG
- Antibody clone number
- 2E9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Temporal and regional patterns of Smad activation in the rat hippocampus following global ischemia.
Nakajima T, Yanagihara M, Nishii H
Journal of the neurological sciences 2014 Feb 15;337(1-2):25-37
Journal of the neurological sciences 2014 Feb 15;337(1-2):25-37
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in Raw 264.7 ( Cat # L024V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SMAD1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
