H00004086-D01P

antibody from Abnova Corporation

Targeting: SMAD1 JV4-1, MADH1, MADR1

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004086-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004086-D01P, RRID:AB_1580760

Product name

SMAD1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human SMAD1 protein.

Antigen sequence

MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVD
ALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRS
LDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKP
LECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLV
PRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDS
FQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSS
DPGSPFQMPADTPPPAYLPPEDPMTQDGSQPMDTN
MMAPPLPSEINRGDVQAVAYEEPKHWCSIVYYELN
NRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLS
NVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSD
SSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNN
QEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGW
GAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMG
SPHNPISSVS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SMAD1 expression in transfected 293T cell line (H00004086-T01) by SMAD1 MaxPab polyclonal antibody.Lane 1: SMAD1 transfected lysate(52.30 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between SMAD1 and GLI3. HeLa cells were stained with anti-SMAD1 rabbit purified polyclonal 1:1200 and anti-GLI3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)