Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB16906 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB16906, RRID:AB_10678084
- Product name
- SMAD1 (phospho S206) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Balancing BMP signaling through integrated inputs into the Smad1 linker.
Sapkota G, Alarcón C, Spagnoli FM, Brivanlou AH, Massagué J
Molecular cell 2007 Feb 9;25(3):441-54
Molecular cell 2007 Feb 9;25(3):441-54
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using SMAD1 (phospho S206) polyclonal antibody (Cat # PAB16906) shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at a 1 : 500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1 : 3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.
