710199

antibody from Invitrogen Antibodies

Targeting: SMAD1 JV4-1, MADH1, MADR1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

710199

Provider

Invitrogen Antibodies

Product name

SMAD1 Recombinant Polyclonal Antibody (12HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

12HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SMAD1 was performed by loading 20 µg of Rat Kidney (lane1) and HUVEC (lane2) lysate using Novex®NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. SMAD1 was detected at ~ 52 kDa using SMAD1 Recombinant Rabbit Polyclonal Antibody (Product # 710199) at 1-3 µg/mL in 2.5% skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG - HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SMAD1 in whole cell extracts of C2C12 using a SMAD1 Recombinant Rabbit Polyclonal Antibody (Product # 710199) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~60kDa.