AP09415PU-N

antibody from Acris Antibodies GmbH

Targeting: SMAD1 JV4-1, MADH1, MADR1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09415PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09415PU-N, RRID:AB_2036120

Product name

anti SMAD1 pSer206

Antibody type

Polyclonal

Antigen

Phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of Human SMAD1 protein.

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Drosophila, Porcine, Sheep

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.51 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity Purified Anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 µg of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reacting with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRPconjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL.