Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-5787-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-NOTCH3 Monoclonal Antibody (MHN3-21), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This MNH3-21 monoclonal antibody reacts with the extracellular domain of human Notch3, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription. Notch3 expression has been demonstrated on some thymocyte subsets, including CD4-CD8- and CD8SP cells. This Notch receptor is also expressed on vascular smooth muscle and cells of the central nervous system. In addition to its role in stem cell hematopoiesis, Notch3 plays a pivotal role in T cell lineage commitment and thymocyte development. Moreover, Notch3 is overexpressed in human T-cell acute lymphoblastic leukemias (T-ALL) and other cancers. Finally, mutation of Notch3 has been linked to cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a CNS degenerative disorder. Applications Reported: This MHN3-21 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MHN3-21 antibody has been pre-titrated and tested by flow cytometric analysis of Jurkat cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MHN3-21
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15.
CADASIL: a critical look at a Notch disease.
Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis.
Combined expression of pTalpha and Notch3 in T cell leukemia identifies the requirement of preTCR for leukemogenesis.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15.
Haraguchi K, Suzuki T, Koyama N, Kumano K, Nakahara F, Matsumoto A, Yokoyama Y, Sakata-Yanagimoto M, Masuda S, Takahashi T, Kamijo A, Takahashi K, Takanashi M, Okuyama Y, Yasutomo K, Sakano S, Yagita H, Kurokawa M, Ogawa S, Chiba S
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6168-78
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6168-78
CADASIL: a critical look at a Notch disease.
Louvi A, Arboleda-Velasquez JF, Artavanis-Tsakonas S
Developmental neuroscience 2006;28(1-2):5-12
Developmental neuroscience 2006;28(1-2):5-12
Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis.
Talora C, Cialfi S, Oliviero C, Palermo R, Pascucci M, Frati L, Vacca A, Gulino A, Screpanti I
Blood 2006 Apr 15;107(8):3313-20
Blood 2006 Apr 15;107(8):3313-20
Combined expression of pTalpha and Notch3 in T cell leukemia identifies the requirement of preTCR for leukemogenesis.
Bellavia D, Campese AF, Checquolo S, Balestri A, Biondi A, Cazzaniga G, Lendahl U, Fehling HJ, Hayday AC, Frati L, von Boehmer H, Gulino A, Screpanti I
Proceedings of the National Academy of Sciences of the United States of America 2002 Mar 19;99(6):3788-93
Proceedings of the National Academy of Sciences of the United States of America 2002 Mar 19;99(6):3788-93
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of Jurkat cells with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human Notch3 APC (purple histogram). Total viable cells were used for analysis.