Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 701975 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOTCH3 Recombinant Rabbit Monoclonal Antibody (4H5L17)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 4H5L17
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), U-87 MG (Lane 2), SH-SY5Y (Lane 3), NTERA-2 (Lane 4), MDA-MB-231 (Lane 5), T-47D (Lane 6), U-2-OS (Lane 7) and A-431 (Lane 8). The blots were probed with Anti-NOTCH3 Recombinant Rabbit Monoclonal Antibody (Product # 701975, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 110 kDa (cleaved fragment) band corresponding to NOTCH3 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with wet transfer method. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).