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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB1559 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Notch-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Notch-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Notch-3 ICD (aa 2195-2321), rhNotch-1, rhNotch-1 ICD (aa 2251-2556), rhNotch-2, rhNotch-2 ICD (aa 2063-2413), rhNotch-4, rhNotch-4 ICD (aa 1778-2003), recombinant mouse Notch-3, or recombinant rat DLL1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9UM47- Isotype
- IgG
- Antibody clone number
- 603532
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
Soriani A, Iannitto ML, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi MR, Petrucci MT, Cippitelli M, Santoni A
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):950-60
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):950-60
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Notch-3 in BG01V Human Stem Cells by Flow Cytometry. BGO1v human embryonic stem cells were stained with Mouse Anti-Human Notch-3 Monoclonal Antibody (Catalog # MAB1559, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
