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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NB100-2414 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2414, RRID:AB_2267121
- Product name
- Goat Polyclonal Notch-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Notch3 signaling initiates choroid plexus tumor formation.
Dang L, Fan X, Chaudhry A, Wang M, Gaiano N, Eberhart CG
Oncogene 2006 Jan 19;25(3):487-91
Oncogene 2006 Jan 19;25(3):487-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Notch-3 Antibody [NB100-2414] - Staining of paraffin embedded Human Hippocampus. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Notch-3 Antibody [NB100-2414] - Staining of paraffin embedded Human Placenta. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Notch-3 Antibody [NB100-2414] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
