CABT-B9284

antibody from Creative Diagnostics

Targeting: PDGFA PDGF-A, PDGF1

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

CABT-B9284

Provider

Creative Diagnostics

Product name

Mouse anti-Human PDGF-AB/BB monoclonal antibody, clone Tjt2

Antibody type

Monoclonal

Description

Platelet derived growth factor (PDGF) plays a role in regulating a number of biological processes including cellular proliferation and development. PDGF consists of a disulfide-linked homo- or heterodimer of two chains, A and B, which are encoded by two separate genes. The three possible combinations of the PDGF chains, AA, AB, and BB, are all biologically active. The PDGF receptor (PDGFR) is a protein tyrosine kinase. Ligand binding to the PDGFR leads to tyrosine phosphorylation of numerous intracellular proteins, including the receptor itself. The PDGFR consists of two subunits, Ï« and ÉÇ, which are encoded by two separate genes. The Ï« subunit binds to both the A and B PDGF chains with high affinity, whereas the ÉÇ subunit binds only to the B chain with high affinity. Ligand binding results in receptor dimerization, with the PDGF type (AA, AB or BB) influencing the resulting PDGFR subunit composition (Ï«Ï«, Ï«ÉÇ, or ÉÇÉÇ). That is, PDGF-AA binds to Ï«Ï« receptors, PDGF-AB binds to Ï«Ï« and Ï«ÉÇ receptors, and PDGF-BB binds to Ï«Ï«, Ï«ÉÇ, and ÉÇÉÇ receptors. This antibody reportedly recognizes B chain-containing human PDGF isoforms and does not recognize the PDGF-AA homodimer. Additionally, this antibody reportedly inhibits PDGFR binding of PDGF-AB and -BB, but not PDGF-AA and is thought to recognize a conformational epitope of the PDGF B chain that is dependent on disulfide linkages. This antibody has been reported to inhibit PDGF receptor-binding and mitogenic effects of PDGF-AB and -BB.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

Tjt2

Vial size

0.1mg

Concentration

0.5 mg/ml

Storage

Store undiluted at 4°C.