Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF1055 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat PDGF-AA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat PDGF-AA in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat (rr) PDGF-AB is observed, 30% cross-reactivity with recombinant human PDGF-AA is observed, and less than 5% cross-reactivity with rrPDGF-BB is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAB59693
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Naïve Schwann Cells in the Sciatic Nerve Injury Model.
Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury.
Zhang M, Jiang MH, Kim DW, Ahn W, Chung E, Son Y, Chi G
BioMed research international 2017;2017:1252851
BioMed research international 2017;2017:1252851
Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury.
Borkham-Kamphorst E, Kovalenko E, van Roeyen CR, Gassler N, Bomble M, Ostendorf T, Floege J, Gressner AM, Weiskirchen R
Laboratory investigation; a journal of technical methods and pathology 2008 Oct;88(10):1090-100
Laboratory investigation; a journal of technical methods and pathology 2008 Oct;88(10):1090-100
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by PDGF-AA and Neutralization by Rat PDGF-AA Antibody. Recombinant Rat PDGF-AA (Catalog # 1055-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat PDGF-AA (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat PDGF-AA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1055). The ND50 is typically 0.2-0.6 µg/mL.