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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-221-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human PDGF-AA Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human PDGF-AA in direct ELISAs and Western blots. Neutralizes the biological activity of human PDGF-AA and will also neutralize the biological activity of recombinant human PDGF-AB, although 2-3 times the amount of IgG is required. It will not neutralize the biological activity of natural human PDGF-AB, porcine PDGF-BB, or rhPDGF-BB.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P04085- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by PDGF-AA and Neutralization by Human PDGF-AA Antibody. Recombinant Human PDGF-AA (Catalog # 221-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-AA Polyclonal Antibody (Catalog # AB-221-NA). The ND50 is typically 5-10 µg/mL.
