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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-23-NA - Provider product page
- Provider
- R&D Systems
- Product name
- PDGF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects PDGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody will recognize recombinant human (rh) PDGF-AB, rhPDGF-AA, rhPDGF-BB, and natural porcine PDGF-BB.
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references In vitro differences between venous and arterial-derived smooth muscle cells: potential modulatory role of decorin.
Differential role of platelet granular mediators in angiogenesis.
Wong AP, Nili N, Strauss BH
Cardiovascular research 2005 Feb 15;65(3):702-10
Cardiovascular research 2005 Feb 15;65(3):702-10
Differential role of platelet granular mediators in angiogenesis.
Brill A, Elinav H, Varon D
Cardiovascular research 2004 Aug 1;63(2):226-35
Cardiovascular research 2004 Aug 1;63(2):226-35
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by PDGF and Neutralization by PDGF Antibody. Human PDGF (Catalog # 120-HD) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Human PDGF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-PDGF Polyclonal Antibody (Catalog # AB-23-NA). The ND50 is typically 3-5 µg/mL.
