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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 675802 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#675802, RRID:AB_2565607
- Product name
- Purified anti-MyD88
- Antibody type
- Monoclonal
- Antigen
- Full length recombinant MyD88 protein produced in 293T cells.
- Description
- MyD88, originally isolated as myeloid differentiation primary response gene, is expressed in a variety of tissues. MyD88 serves as a cytoplasmic adaptor protein involved in the signaling of TLR and IL-1R family members. It associates with and recruits IRAK to IL-1R complex in response to IL-1. This pathway leads to activation of NF-kB. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18. MyD88 deficient mice lack a response to LPS (a bacterial product) which employs toll like receptors 2 and 4 (TLR2 and TLR4) as signaling receptors.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- O91B8
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot analysis of THP-1 (lane 1), Raw264.7 (lane 2) and Jurkat (lane 3). Anti-MyD88 antibody (clone O91B8) was used, followed by HRP anti-mouse IgG antibody (Cat. No. 405306). β-actin antibody (Poly6221) was used as a loading control.
- Conjugate
- Unconjugated
